Beijing Hotgen Biotech Co.,Ltd.

Hotgen  Heart-type Fatty Acid Binding Protein (H-FABP) Test Cassette

Hotgen  H-FABP kit is an accurate and rapid immunochromatografic test for the  qualitative determination of Heart-type Fatty Acid Binding Protein (H-FABP) in  human serum and plasma as an aiding tool for early diagnosis of cardiac  infarction.

H-FABP:  An early, specific marker for cardiac infarction

Heart-type  Fatty Acid-Binding Protein (H-FABP) is a small cytoplasmic protein (15 kDa)  released from cardiac myocytes very rapidly following Acute Myocardial  Infarction (AMI).

The  diagnostic potential of the biomarker H-FABP for heart injury was discovered in  1988 by Professor Jan Glatz (Maastricht, Netherlands). H-FABP concentrations  typically peak at approximately 6-8 hours after onset of chest pain and return  to normal within 24-30 hours. Although H-FABP has similar release kinetics to  Myoglobin (i.e. rapid release & clearance), H-FABP is 20 times more specific  to cardiac muscle than myoglobin,   it is found at 10-fold lower levels in skeletal muscle than heart muscle and  the amounts in the kidney, liver and small intestine are even lower again, hence  making it a vastly more efficacious biomarker for myocardial injury.  

Furthermore,  H-FABP has been repeatedly shown to be a highly sensitive early rise biomarker  across the full spectrum of acute coronary syndrome (ACS), detectable as early  as 30 minutes following the onset of an ischemic episode.

H-FABP:  Release Kinetics

A  secondary benefit of the H-FABP release kinetics after AMI is that since it  returns to baseline concentrations so rapidly (i.e. typically 20-24 hours), it  can be used as a biomarker for recurrence of infarction in the days following an  initial AMI. This has been a common utility of CK-MB historically, due to its  more rapid clearance than with Troponin (3-4 days vs 10-14 days), but H-FABP  offers potential use from as early as the second day  onwards.

H-FABP  in Reinfarction

In  addition to its diagnostic potential, H-FABP also has prognostic value.  Alongside D-dimer, NT-proBNP and peak troponin T, studies show that it was the  only cardiac biomarker that proved to be a statistically significant predictor  of death or MI at one year. This prognostic information was independent of  troponin T, ECG and clinical examination.

H-FABP  is a very stable protein in-vitro, as studies have shown that serum and plasma  samples can be subjected to up to 8 freeze/thaw cycles without the loss of  immunoreactivity.

 Benefits  of H-FABP test in summary:

Early,  sensitive, and specific biomarker for MI

Early  and sensitive biomarker for ACS

Ideal  biomarker for recurrence of heart injury

Independent  cardiac biomarker predicting risk of MI

Recommended  to be tested in combination with Troponin I to increase the sensitivity and  specificity

Hotgen Heart-type  Fatty Acid Binding Protein Test Principle

Colloidal  gold immunochromatographic method

Hotgen Heart-type  Fatty Acid Binding Protein Kit Features

Qualitative  H-FABP measurement

Rapid,  accurate, lab quality results within minutes

Ready-to-use  reagents

Long  shelf life

Easy  storage at room temperature

Single  dose format: run only the test you need